| Rose Bengal Brucella Antigen. |
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| Rose Bengal antigen is a colored Brucellla abotus cells (strain 99)for rapid diagnosis of Brucellosis in animals |
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| Composition : |
| This antigen is prepared from heat killed brucella abortus cells (strain 99) stained with rose Bengal strain and kept in a stable acidic suspension 3.65. |
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| Uses |
| Rose Bengal antigen is used for rapid detection of Brucellosis in animals (as cattle, buffaloe sheep and goat) by rapid agglutination test. |
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| Methodology :(principle of the procedure) |
| 1- One drop from the antigen (0.03 ml) is mixed with equal |
| amount of the tested serum on a ceramic plate using |
| tooth pick or a glass rod. |
| 2- put the plate on the rocker for 4 minutes. |
| 3- Read the results as follow : |
| No agglutination negative (-ve) |
| Weak agglutination (minimal reaction) ± |
| Slight agglutination after 4 min + |
| Moderate agglutination within 4 min ++ |
| Moderate agglutination immediately +++ |
| Clear agglutination immediately ++++ |
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| Precautions : |
| - The test must be performed in a clean place far from dust. |
| - The antigen bottle must be shaken before use. |
| - The used antigen and the tested serum must take the room temperature before use. |
| - Control positive and control negative serum have to be |
| used to confirm the results. |
| - Read the results, within 4 min. only. |
| - Materials used in the test as tooth pick, gloves or others should be disposed as potentially biohozardous waste. |
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| Storage: |
| - This product must be kept at 4-8oC . |
| - Don’t freeze. |
| |
| Expire: |
| Two years from production date. |
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| Package: |
| Vials contains 20 ml ( 600diagnostic units). |
| Vials contains 10 ml ( 300diagnostic units). |
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