Rose Bengal Brucella Antigen. |
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Rose Bengal antigen is a colored Brucellla abotus cells (strain 99)for rapid diagnosis of Brucellosis in animals |
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Composition : |
This antigen is prepared from heat killed brucella abortus cells (strain 99) stained with rose Bengal strain and kept in a stable acidic suspension 3.65. |
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Uses |
Rose Bengal antigen is used for rapid detection of Brucellosis in animals (as cattle, buffaloe sheep and goat) by rapid agglutination test. |
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Methodology :(principle of the procedure) |
1- One drop from the antigen (0.03 ml) is mixed with equal |
amount of the tested serum on a ceramic plate using |
tooth pick or a glass rod. |
2- put the plate on the rocker for 4 minutes. |
3- Read the results as follow : |
No agglutination negative (-ve) |
Weak agglutination (minimal reaction) ± |
Slight agglutination after 4 min + |
Moderate agglutination within 4 min ++ |
Moderate agglutination immediately +++ |
Clear agglutination immediately ++++ |
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Precautions : |
- The test must be performed in a clean place far from dust. |
- The antigen bottle must be shaken before use. |
- The used antigen and the tested serum must take the room temperature before use. |
- Control positive and control negative serum have to be |
used to confirm the results. |
- Read the results, within 4 min. only. |
- Materials used in the test as tooth pick, gloves or others should be disposed as potentially biohozardous waste. |
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Storage: |
- This product must be kept at 4-8oC . |
- Don’t freeze. |
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Expire: |
Two years from production date. |
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Package: |
Vials contains 20 ml ( 600diagnostic units). |
Vials contains 10 ml ( 300diagnostic units). |
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